Table 2.
CCoV RNA titres in the organs of dogs succumbed to natural or experimental infection with strain CB/05
| 117/05-Aa | 117/05-Ba | 117/05-Ca | Dog 1b | Dog 3b | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Tissue/sample | RRT-PCR | VI | RRT-PCR | VI | RRT-PCR | VI | RRT-PCR | VI | RRT-PCR | VI |
| Faeces | 6.12 × 105 | + | 9.24 × 104 | + | 3.17 × 105 | + | 5.14 × 104 | + | 1.09 × 105 | + |
| Lung | 1.08 × 106 | + | 2.74 × 104 | + | 2.32 × 106 | + | 6.79 × 103 | − | 2.99 × 104 | + |
| Spleen | 4.46 × 106 | + | 1.20 × 105 | + | 6.87 × 106 | + | 5.48 × 104 | + | 3.07 × 104 | + |
| Liver | 9.02 × 104 | + | 5.98 × 104 | + | 3.12 × 105 | + | 1.93 × 104 | + | 4.70 × 103 | − |
| Kidney | 7.54 × 105 | + | 1.40 × 104 | + | 2.53 × 105 | + | 5.11 × 103 | − | 2.94 × 103 | − |
| Mesenteric lymph node | 8.09 × 104 | + | 2.01 × 104 | + | 5.17 × 104 | + | 2.19 × 104 | − | 3.61 × 104 | + |
| Brain | 5.23 × 103 | − | 9.52 × 102 | − | 1.25 × 104 | − | 3.32 × 102 | − | 6.00 × 102 | − |
RRT-PCR, real-time RT-PCR. Results are expressed as CCoV type II RNA copy number/μl of template. VI, virus isolation. Results are expressed as positive (+) or negative (−).
Dogs succumbed to natural infection (described by Decaro et al., 2007b).
Dogs euthanized after experimental infection (this study).