Skip to main content
. 2009 Nov 6;142(3):261–267. doi: 10.1016/j.vetmic.2009.10.023

Fig. 2.

Fig. 2

Each of siRNAs co-transfected with pEGFP-ORF2/pEGFP in A549 cells. The expression of HEV ORF2 protein was observed 48 h post-transfection. HEV specific siRNAs induced an obvious reduction, whereas the expression level of EGFP protein co-transfected with scrambled siRNA showed no significant change. Each of siRNAs (si-ORF2-1 or si-ORF2-2) was co-transfected with pEGFP vector to test the interferon response. pEGFP-ORF2: pEGFP-ORF2 recombinant plasmid transfection alone; pEGFP+ si-ORF2-1: pEGFP vector co-transfected with si-ORF2-1; pEGFP+ si-ORF2-2: pEGFP vector co-transfected with si-ORF2-2; scrambled siRNA: pEGFP-ORF2 recombinant plasmid co-transfection with scrambled siRNA; si-ORF2-1: pEGFP-ORF2 recombinant plasmid and si-ORF2-1 co-transfection; si-ORF2-2: pEGFP-ORF2 recombinant plasmid and si-ORF2-2 co-transfection; si-ORF2-3: pEGFP-ORF2 recombinant plasmid and si-ORF2-3 co-transfection; si-ORF2-4: pEGFP-ORF2 recombinant plasmid and si-ORF2-4 co-transfection. Pictures were taken at 48 h post-transfection with a Nikon TE2000 fluorescence microscope.