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Inhibitory effect of HEV protein expression by siRNAs. Western blot was performed on equal amounts of protein harvested from transfected HEV specific siRNAs (10 nM) A549 cells at 72 h post-inoculation. GAPDH was used as a loading control. M: Marker; HEV: HEV inoculated alone; scrambled: scrambled siRNA transfection; si-ORF2-1: si-ORF2-1 transfection; si-ORF2-2: si-ORF2-2 transfection; si-ORF2-3: si-ORF2-3 transfection; si-ORF2-4: si-ORF2-4 transfection.
