Fig. 6.

Formation of FMDV empty capsids in HF cells containing recombinant baculovirus Bac-P12A3C. (A) Concentrated lysates and supernatants from HF cells infected with recombinant baculovirus Bac-P12A3C were treated and centrifuged on a 15–45% (wt/vol) sucrose gradient as described in Section 2. FMDV-infected BHK cells were centrifuged on a parallel gradient and used as a marker. Samples from each fraction were assayed by spectrophotometry. (B) Electron microscopy images of FMDV 75S empty capsids from FMDV-infected BHK cells. (C) Electron microscopy images of empty capsid-like particles from recombinant baculovirus Bac-P12A3C-infected HF cells.