Table 1.

The list of the oligonucleotide primers designed for detecting and sequencing.

Target virusesa	Target geneb	Primer sequence, 5′ to 3′c	Region (nt)	Size (bp)
GARV	VP6	F: AAA GAT GCT AGG GAC AAA ATT G	58–78	308
		R: TTC AGA TTG TGG AGC TAT TCC A	344–365
		nF: GAC AAA ATT GTC GAA GGC ACA TTA TA	69–94	121
		nR: TCG GTA GAT TAC CAA TTC CTC CAG	166–189
	VP4	F: GCT TCG CTC ATT TAT AGA CA	12–31	877
		R: ATT TCG GAC CAT TTA TAA CC	868–887
	VP7	F: GGC TTT AAA AGA GAG AAT TTC	1–21	1062
		R: GGT CAC ATC ATA CAA TTC TAA	1042–1062
GBRV	NSP2	F: CTA TTC AGT GTG TCG TGA GAG G	18–40	434
		R: GCA GAC AAG CTA GCC CGC TTC G	429–451
GCRV	VP6	F: CTC GAT GCT ACT ACA GAA TCA G	994–1018	366
		R: AGC CAC ATA GTT CAC ATT TCA TCC	1339–1359
		nF: CTC GAT GCT ACT ACA GAA TCA G	994–1018	328
		nR: GGG ATC ATC CAC GTC ATG CGT	1300–1321
PSaV	RdRp	F: GTG CTC TAT TGC CTG GAC TA	4312–4331	572
		R: TCT GTG GTG CGG TTA GCC TT	4864–4883
		nF: GTG GTA TGC TGA GGA CAC AC	4392–4411	380
		nR: GAG TGT CTG TTG GCT CAA TG	4752–4771
PSaV&	RdRp	F: GAT TAC TCC AAG TGG GAC TCC AC	4568–4590	319
PNoV		R: TGACAA TGT AAT ATC ACC ATA	4865–4886
PToV	N	F: GTCAGAATAGATCACGCATT	170–189	185
		R: CGCCAAACTCTGCAACTCAGGTGGA	330–354
TGEVd	ORF1b	F GGG TAA GTT GCT CAT TAG AAA TAA TGG	7968–7994	1006
	Spike	R: CTT CTT CAA AGC TAG GGA CTG	920–940
PEDV	N	F: AGG AAC GTG ACC TCA AAG ACA TCC C	812–836	540
		R: CCA GGA TAA GCC GGT CTA ACA TTG	1328–1351

^aGARV: group A rotavirus; GBRV: group B rotavirus; GCRV: group C rotavirus; PSaV: porcine sapovirus; PNoV: porcine norovirus; PToV: porcine torovirus; TGE: transmissible gastroenteritis coronavirus; PED: porcine epidemic diarrhea coronavirus.

^bRdRp: RNA dependent RNA polymerase; ORF: open reading frame; N: nucleocapsid.

^cF: upstream primer for RT-PCR; R: downstream primer for RT-PCR; nF: upstream primer for nested PCR; nR: downstream primer for nested PCR.

^dTGEV: forward primer was designed from the portion of TGEV ORF1b; reverse primer was designed from the portion of TGEV spike gene.