Table 1.

Detection of PED virus in a faecal sample from an experimentally infected SPF piglet using three variants of monoclonal blocking ELISA methods

ELISA variant	Dilution of faces	Results obtained with different dilutions of faeces and swine sera
		SwS dilution 10×				SwS dilution 160×
		Neg.	Pos.	NA	%B	Neg.	Pos.	NA	%B
(1) DAS-ELISA mAbPEDVa HRPO-mAbPEDVb	2×	1.012	0.161	0.851	84.1	1.120	1.001	0.119	10.6
	8×	0.420	0.123	0.297	70.7	0.657	0.470	0.187	28.5
	32×	0.190	0.119	0.071	37.4	0.203	0.183	0.020	9.9
	128×	0.155	0.174	−0.019	−12.2	0.153	0.193	−0.040	−26.1
(2) CB-ELISA SwAPEDVa HRPO-SwAMoIgb	2×	1.531	0.094	1.437	93.3	1.382	1.012	0.370	26.8
	8×	0.762	0.066	0.696	91.3	1.382	0.582	0.800	57.9
	32×	0.246	0.063	0.183	74.4	0.992	0.143	0.849	85.6
	128×	0.126	0.068	0.058	46.0	0.250	0.112	0.138	55.2
(3) DAS-ELISA SwAPEDVa HRPO-mAbPEDVb	2×	1.131	0.171	0.960	84.9	1.068	0.868	0.200	18.7
	8×	0.340	0.120	0.220	64.7	0.890	0.484	0.405	45.5
	32×	0.142	0.123	0.019	13.4	0.468	0.241	0.227	48.5
	128×	0.157	0.121	0.037	22.9	0.247	0.171	0.076	30.8

Pairs of microtitre plate wells precoated with PEDV binding antibodies were incubated with mixtures containing a faecal sample from a piglet 48 hpi diluted 1:2 to 1:128 and swine PEDV negative or positive serum diluted 1:10 and 1:160; NA: Net absorbance. Differences of mean absorbance in wells incubated with SwSneg.–SwSpos.; %B: reduction of absorbance in the wells incubated with SwSpos. in comparison with the wells incubated with SwSneg.; Bold letters indicate dilutions at which positive results were obtained (NA > 0.1 and %B > 50).

^aBinding antibodies.

^bDetection antibodies (conjugate).