Table 2.

Sensitivity and specificity of PEDV detection in control V-Ag samples and in field samples of faeces by the CB-ELISA method

Group of samples	Sample tested	EM	Dilution of samples		Absorbances in wells		NA	%B	E
			Ag/faeces	SwSneg./pos.	SwSneg.	SwSpos.
1	PED V-Ag	C+	40×	40×	1.822	0.070	1.752	96.2	+
	TGE V-Ag	C+	40×	40×	0.025	0.018	0.007	28.0	−
	Rota V-Ag	R+	40×	40×	0.024	0.039	−0.015	−62.5	−
2	676/2	C+	2×	40×	0.503	0.050	0.453	90.1	+
	716/2	C−	2×	40×	0.728	0.295	0.433	59.5	+
	726/3	C−	2×	40×	1.652	0.282	1.370	82.9	+
3	708/3	C−	2×	40×	1.765	1.210	0.555	31.4	−
	708/3	C−	2×	20×	1.671	0.506	1.165	69.7	+
	829/2	C−	2×	40×	2.104	1.147	0.957	45.5	−
	829/2	C−	2×	20×	1.792	0.267	1.525	85.1	+
4	771/4	C+	2×	40×	0.094	0.041	0.053	56.4	−
	771/4	C+	2×	500×	0.899	0.184	0.715	79.5	+
	837	ND	2×	40×	0.076	0.045	0.031	40.8	−
	837	ND	2×	500×	0.296	0.016	0.280	94.6	+
5	833/2	C−	2×	40×	0.120	0.070	0.050	41.7	−
	833/2	C−	2×	500×	0.038	0.047	−0.009	−23.7	−
6	663/3	C−	2×	40×	0.090	0.085	0.005	5.6	−
	668	C−	2×	40×	0.016	0.017	−0.001	−6.3	−

Effect of SwSneg./pos. dilution on the values of net absorbance, % blocking and final evaluation. Dilutions of samples with positive values NA > 0.1 and %B > 50 are in bold; EM (+/−): positive and negative results of rotavirus (R) and coronavirus (C) detection obtained by electron microscopic examination; NA: net absorbance; %B: percentage of reaction blocking; E: evaluation of samples by CB-ELISA as PEDV positive (+) or negative (−); ND: not done; PED, TGE, Rota V-Ag: mean absorbance obtained by examination of crude viral antigens.