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. 2019 Nov 20;240:108513. doi: 10.1016/j.vetmic.2019.108513

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© 2019 Elsevier B.V. All rights reserved.

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Fig. 5 — Infection of chickens with PhCoV isolates I0623/17 and I0710/17 and of pheasants with I0710/17. Virus recovery from oropharyngeal (I) and cloacal (II) swabs from chickens inoculated with PhCoV isolates I0623/17 and I0710/17, and pheasants with PhCoV isolate I0710/17 (A). Virus recovery was performed by inoculating 9-day-old embryonated, specific pathogen-free eggs through the allantoic route with supernatant from the swabs. Replication of our isolates I0623/17 and I0710/17 in the trachea, lung, proventriculus, cecal tonsil, and kidney of chickens at 5 dpi, and replication of I0710/17 in the trachea, lung, proventriculus, cecal tonsil, and kidney of pheasants at 5 dpi (B). Viral titration was performed by inoculating 9-day-old embryonated, specific pathogen-free eggs through the allantoic route. Data are expressed as the mean ± standard deviation. Virus titers were analyzed by Student’s t-test using GraphPad Prism for Windows version 5. Differences were considered significant if the p value was <0.05 (*p < 0.05, **p < 0.01, ***p < 0.001). Detection of the IBV antigen by IHC analysis in the kidney (I) and trachea (II) of pheasants at 5 dpi following PhCoV I0710/17 infection and in the trachea (III) of chickens at 5 dpi following PhCoV I0623/17 infection (C).

Fig. 5 — Infection of chickens with PhCoV isolates I0623/17 and I0710/17 and of pheasants with I0710/17. Virus recovery from oropharyngeal (I) and cloacal (II) swabs from chickens inoculated with PhCoV isolates I0623/17 and I0710/17, and pheasants with PhCoV isolate I0710/17 (A). Virus recovery was performed by inoculating 9-day-old embryonated, specific pathogen-free eggs through the allantoic route with supernatant from the swabs. Replication of our isolates I0623/17 and I0710/17 in the trachea, lung, proventriculus, cecal tonsil, and kidney of chickens at 5 dpi, and replication of I0710/17 in the trachea, lung, proventriculus, cecal tonsil, and kidney of pheasants at 5 dpi (B). Viral titration was performed by inoculating 9-day-old embryonated, specific pathogen-free eggs through the allantoic route. Data are expressed as the mean ± standard deviation. Virus titers were analyzed by Student’s t-test using GraphPad Prism for Windows version 5. Differences were considered significant if the p value was <0.05 (*p < 0.05, **p < 0.01, ***p < 0.001). Detection of the IBV antigen by IHC analysis in the kidney (I) and trachea (II) of pheasants at 5 dpi following PhCoV I0710/17 infection and in the trachea (III) of chickens at 5 dpi following PhCoV I0623/17 infection (C).