Table 3.
Summary of the RT-PCR and nested PCR assay results for the individual swine fecal samples
| Period | Porcine fecal samples positive by using different PCR assaysa (no. of positive/total no. of samples [%]) |
No. of total positive samplesb | ||||
|---|---|---|---|---|---|---|
| RT-PCR P289/290 | RT-PCR PEC45/46 | Nested PCRc PECnF/nR | RT-PCR CapF/R | Nested PCRc CapnF/nR | ||
| 2004 | 4/80 (5%) | 3/80 (3.8%) | 23/80 (28.8%) | 8/80 (10.0%) | 11/80 (13.8%) | 26/80 (32.5%) |
| 2005 | 11/157 (7.1%) | 2/157 (1.3%) | 27/157 (17.2%) | 14/157 (8.9%) | 28/157 (17.8%) | 43/157 (27.3%) |
| Total | 15/237 (6.3%) | 5/237 (2.1%) | 50/237 (21.1%) | 22/237 (9.3%) | 39/237 (16.5%) | 69/237 (29.1%) |
a
Virus and target proteins for each primer pair are listed in Table 1.
b
Fecal samples were considered positive if at least one positive fecal sample was detected in the same sample by each primer pair.
c
The number of nested PCR positive samples also contained the number of RT-PCR positive samples.