Table 1.

Scoring system for general health observations during the challenge trial.

Parameter	Result	Score
General appearance	Normal	0
	Depressed/lethargic	2
	Difficulties in breathing	3
	Death	20
Dehydration	None (<4%): not detectable	0
	Mild (4–5%): subtle loss of skin elasticity	1
	Moderate (6–8%): definite delay in return of skin to normal position; eyes possibly sunken in orbits; slightly prolonged capillary refill time; possibly dry mucous membranes	2
	Severe (10–15%): tented skin standing in place; prolonged capillary refill time; eyes sunken in orbits; dry mucous membranes possible signs of shock (increased heart rate, weak pulses); death imminent	3
Diarrhoea	None	0
	Soft	1
	Liquid	2
	Bloody	3
Rectal temperature	37.0–39.4 °C	0
	≥39.5 °C	2
	<37.0 °C	3
Body weight	Weight loss	2
	No weight loss	1
	Weight gain	0