Fig. 4.

Cholesterol depletion did not reduce attachment of BoHV-1. (a) Binding of BoHV-1 to the target cells was determined with virus titration. MDBK cells were mock treated or were treated with 5 and 10 mM MβCD for 30 min, and then binding of BoHV-1 to the cells was processed at 4 °C for 1h. The amount of cell-associated virus was titrated in MDBK cells. (b) Binding of BoHV-1 to the target cells was determined with FACS. Cells were mock treated or treated with 10 mM MβCD and then infected or mock infected with BoHV-1 at 4 °C. At 1h later, the cells were fixed with 4% paraformaldehyde and stained with bovine anti-BoHV-1 serum followed by FITC-conjugated anti-bovine immunoglobulin. Experiments were repeated three times, and the error bars indicate the standard deviations of three independent experiments. Image is one of three independent experiments.