Fig. 5.

Viral envelope cholesterol was required for the virus infectivity. (a) Infectivity of BoHV-1 which was mock treated or treated with various concentrations of MβCD, was measured by plaque formation assay. (b) Infectivity of BoHV-1 which was replenished with exogenous cholesterol was measured by plaque formation assay. Cell was first mock pretreated or pretreated with 10 mM MβCD and then mock replenished or replenished with 400 μg/ml cholesterol for 1 h before BoHV-1 infection. Experiments were repeated three times, and the error bars indicate the standard deviations of three independent experiments.