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Chronic shedding of feline coronavirus as monitored by RT-PCR on feces. Amplification reactions were targeted to the 3’ nontranslated region of the viral genome. Cats H324 and H419 were placed in isolation at Day 0. The numbers of days that the cats were in isolation is on the horizontal axis. The RT-PCR results are expressed by bars above (i.e. positive in RT-PCR) or below (i.e. negative) the horizontal axis. Long and short bars represent single and nested RT-PCR.
