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. 2018 Apr 22;219:226–233. doi: 10.1016/j.vetmic.2018.04.026

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© 2018 The Authors

Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.

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Fig. 1 — A. Molecular structure of triphosphorylated GS-441524. B. CRFK cell cultures infected with FIPV-79-1146 showing typical CPE and crystal violet staining pattern. Infected cells were treated with 3 uM (row a), 2 uM (row b), 1 uM (row c), 0.75 uM (row d), 0.5 uM (row e), 0.25 uM (row f), 0 uM GS-441524 (row g); untreated and uninfected cells (row h). C. A nonlinear regression analysis utilizing these data determined the EC50 for GS-441524 to be 0.78 uM.