Fig. 2.

Rectal temperatures and neutralizing antibody titres. (A) Rectal temperatures were taken daily for the first 14 days post vaccination/challenge and at 21, 28, 55, and 61 dpv. (B) Neutralizing antibody (NA) titres in serum were determined using a virus neutralization assay for samples obtained every other day during the first 14 days post vaccination/challenge and at 21, 28, 55, and 61 dpv. White, black, and grey circles represent data from animals in Group 1, 2, and 3, respectively. The days of vaccination (0) and challenge (34) are depicted in bold face. The dotted line in panel B represents the limit of detection. Measurements/samples taken on the day of vaccination/challenge were obtained before infection and horses in Group 3 were included in the experiment starting at the day of challenge. Abbreviations: DUB+, deubiquitinase-competent vaccine virus; DUB−, deubiquitinase-negative vaccine virus; NA, neutralizing antibody.