Table 1.

RT-PCRs for the differentiation between feline coronavirus (FCoV) type I and FCoV type II/canine coronavirus (CCV)

Primer name	Sequence	Orientation	Length of the amplified product (bp)	Annealing temperature (°C)
FCoV type I (RT-PCR, Benetka et al., 2004)
Fecv1bf	5′-ttgaccttgactggctcaac-3′	Sense	275	60
Fecv1br	5′-cgtccacagagatgccaata-3′	Antisense
FCoV type II (RT-PCR, Benetka et al., 2004)
Fecv2bf	5′-aggttgttgtggatgcatag-3′	Sense	232	60
Fecv2br	5′-acggtcaagttcgtcaagta-3′	Antisense
FCoV type I (RT-PCR, Posch et al., 2001)
FCoV type I f	5′-cctgtaccatcgtggtctaa-3′	Sense	340	48
FCoV type I r	5′-ctcgaacagttggtggaagt-3′	Antisense
FCoV type II (RT-PCR, Posch et al., 2001)
FCoV type II f	5′-gtgccatgattgtgctcgta-3′	Sense	661	48
FCoV type II r	5′-gcagtgcttgagcgtgaata-3′	Antisense