Table 2.

Reaction schemes for differentiating RT-PCRs

Reaction	Reaction volume (μl)	Reaction scheme	Number of cycles
Reverse transcription	25 and 20, respectively	50 °C, 30 min; 95 °C, 15 min	1
PCR, Benetka et al. (2004)	25	94 °C, 30 s; 60 °C, 30 s; 72 °C, 30 s	40
PCR, Posch et al. (2001)	20	94 °C, 30 s; 50 °C, 1 min; 72 °C, 30 s	45