Fig. 6.

Effect of bile and bile acids on the infection of PEDV icPC22A and icPC22A-S1Δ197 in Vero (A–E) and IPEC-DQ cells (F–J). Monolayers of Vero or IPEC-DQ cells in 96-well plates were inoculated with a MOI of 0.02 icPC22 A or icPC22 A-S1Δ197 in medium containing 10 μg/mL trypsin and different concentrations of bile or bile acids. After incubation at 37℃ for 2 h, the inoculum was removed followed by three washings. DMEM/RPMI-1640 containing 50 μg/mL SBTI and swine PEDV positive serum (virus neutralization titer 1:1024, 1:500 dilution) was added. At 8 hpi, cells were fixed with acetone-methanol (1:4) and infectivity was tested by IF assay. Each Experiment was performed three times. Data are shown as M ± SD, and number of asterisks indicate significant difference between groups (*, p < 0.05; **, P < 0.01; ***, P < 0.001).