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. 2018 Nov 27;228:202–212. doi: 10.1016/j.vetmic.2018.11.025

Table 1.

Primers and probes of the duplex RT-qPCR.

Name Nucleotide sequence (5’-3’)
PC22A-21220F (for icPC22A) GTTACAACAGTGGAGGTTGT
PC22A-21280FP (for icPC22A) CY5-ATGTTACTAGTGCTGGTGAGGATGGT-BHQ2
PC22A-20678F (for icPC22A-S1Δ197) AACACTTAGCCTACCACAA
PC177-P2-20698FP (for icPC22A-S1Δ197) FAM-TGCTCAGCTAACACTAATTTTAGGACAGCT-BHQ3
PC22A-21374R (for both viruses) ATTGGGCTCAGTAGCAAAT