Fig. 3.

Knockdown of eIF3i by siRNAs effect VSV growth.

(A) HeLa cells were transfected with 50 nM control siRNA or three different siRNAs targeting eIF3i. At 48 h post-transfection, the levels of the eIF3i protein were determined by immunoblotting with an anti-eIF3i polyclonal antibody (catalog no. 11287-1-AP; Proteintech). Actin served as the loading control. (B and C) HeLa cells were transfected with 50 nM control siRNA or sieIF3i 2. Subsequently, the cells were infected with VSV (MOI = 1). At 4, 8 and 12 h post-infection, VSV P mRNA (B) and VSV antigenomic RNA (C) levels were determined by qRT-PCR. (D) HeLa cells were transfected with 50 nM control siRNA or sieIF3i 2. Subsequently, the cells were infected with VSV (MOI = 1). At 4, 8 and 12 h post-infection, virus titers were examined by TCID₅₀ assay. The data were averaged from three replicates of three independent experiments. The data are shown as the means ± SD (***p < 0.01; **p < 0.05; n.s., no significance).