Fig. 6.

EIF3I may affect VSV growth by modulating host antiviral response in HeLa cells.

(A) VSV inhibited Akt phosphorylation at threonine 308. HeLa cells were infected or mock infected with VSV (MOI = 10). The level of Akt phosphorylation was determined by immunoblotting with an anti-phospho-Akt (Thr308) mAb (catalog no. 4056; Cell Signaling Technology). (B–D) Knockdown of eIF3i inhibited the expression of Akt/EMSY-regulated ISGs after infected with VSV. HeLa cells were transfected with 50 nM control siRNA or sieIF3i 2. At 50 h post-transfection, cells were infected with VSV (MOI = 1). At 4, 8 and 12 h post-infection, ISG15 (B), IFITM1 (C) and Viperin (D) mRNA were determined by qRT-PCR. (E) VSV did not affect the expression of eIF3i protein. HeLa cells were infected or mock infected with VSV at an MOI of 10. The level of eIF3i protein was determined by immunoblotting with an anti-eIF3i polyclonal antibody (catalog no. 11287-1-AP; Proteintech). Each experiment was repeated at least three times. The data are shown as the means ± SD (***p < 0.01; **p < 0.05).