Fig. 2.

CCoV infection induces cell death in A-72 canine cell line. Upper panels: 48 h after infection (p.i.) with CCoV (moi 0.1), cells were examined for cytopathic effect under phase contrast microscope. Lower panels: nuclear staining with Hoechst 33324. A-72 cell line infected with CCoV (48 h p.i.) showing nuclear condensation and fragmentation with typical apoptotic bodies (indicated by arrow). Control label indicates parallel uninfected A-72 cell culture.