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. 2006 Dec 28;121(1):64–72. doi: 10.1016/j.vetmic.2006.12.016

Fig. 6.

Fig. 6

Caspase-3 activation by CCoV infection. (A) Caspase activity was measured by colorimetric assay in control (A-72 control) and CCoV infected cells untreated (A-72 CCoV) or treated with caspase-3 inhibitor ZVAD (A-72 CCoV + ZVAD). The results are expressed as the means of two independent experiments. (B) Proteolytic cleavage of procaspase-3 was detected by immunoblotting of 50 μg of cell lysates from uninfected A-72 cell line (c) or CCoV infected cells (i) obtained at indicated times p.i. (30 and 48 h). Apoptotic Jurkat cells treated with anti-Fas mAb was included in this analysis as positive control for procaspase-3 cleavage.