Table 2.

Dicer-substrate siRNA sequences. RNA bases are uppercase, DNA bases are lower case. Previously tested siRNA sequences are shown shaded in grey (the central 19mer duplex). Dicer-substrate siRNAs designed based on the reported sequence of FCoV FIPV1146 (accession number DQ010921).

DsiRNA	Sequence
Leader (DsiRNA-L2)
Sense	5′-GGACACCAACUCGAACUAAACGAaa-3′
Antisense	3′-AGCCUGUGGUUGAGCUUGAUUUGCUUU-5′
Nucleocapsid (DsiRNA-N1)
Sense	5′-GGAGUCUUCUGGGUUGCAAGGGAtg-3′
Antisense	3′-UACCUCAGAAGACCCAACGUUCCCUAC-5′
NSC-GFP (DsiRNA-NSC)
Sense	5′-CAAGCUGACCCUGAAGUUCAUCUgc-3′
Antisense	3′-CCGUUCGACUGGGACUUCAAGUAGACG-5′