Table 1.
Porcine epidemic diarrhea virus (PEDV) titer of inoculated liquid and spray-dried bovine plasmaa.
| Spray drying condition | Inoculated liquid plasma PEDV TCID50/mL |
Spray-dried plasma PEDV TCID50/g |
|---|---|---|
| 70 °C throughout substance | 4.2 ± 0.2 log10 (Ct: 13.9 ± 0.3) | Not detected (Ct: 23.3 ± 0.6)b,c |
| 80 °C throughout substance | 4.2 ± 0.2 log10 (Ct: 13.9 ± 0.3) | Not detected (Ct: 23.8 ± 0.8) |
a
Data represents the mean PED virus isolation results of triplicate analysis of three lots per liquid and spray dried condition that had been subjected to three serial passages on VERO cell monolayers.
b
The spray dryer concentrated liquid plasma 10 times; the expected virus titer in the dry product was 5.15 ± 0.2 log10 TCID50/g.
c
The theoretical limit of detection of the method used was estimated to be able to detect as low as 0.7 viral particles/g.