Table 2.

Porcine epidemic diarrhea virus (PEDV) titer of inoculated spray-dried bovine plasma samples held at various time by temperature conditions^a.

Temp.	Time	Ct valuesb	PEDV titer (TCID50/g) per sample numberc
(°C)	(d)	(mean ± SD)	1	2	3	4	5
−80	0	22.0 ± 0.8	Average 2.8 log10
4 ± 2	7	21.6 ± 0.7a	0.8 log10	0.8 log10	0.8 log10	0.93 log10	Neg
4 ± 2	14	30.9 ± 0.6b	Neg	Neg	Neg	0.8 log10	Neg
4 ± 2	21	31.5 ± 0.3c	Neg	Neg	Neg	Neg	Neg
12 ± 2	7	22.2 ± 1.1a	Neg	Neg	Neg	Neg	0.8 log10
12 ± 2	14	24.3 ± 1.0b	Neg	Neg	Neg	Neg	Neg
12 ± 2	21	31.1 ± 0.3c	Neg	Neg	Neg	Neg	Neg
22 ± 2	7	21.1 ± 0.6a	Neg	Neg	Neg	Neg	Neg
22 ± 2	14	32.6 ± 1.3b	Neg	Neg	Neg	Neg	Neg
22 ± 2	21	34.8 ± 2.0c	Neg	Neg	Neg	Neg	Neg

^aThe theoretical limit of detection of the PED virus isolation method used was estimated to be able to detect as low as 1.0 viral particles/g.

^bPEDV Ct values with uncommon superscripts within temperature by time storage condition differ (P < 0.05).

^cNeg = negative or not detectable.