Table 1.
Character of each selected epitope and used primers in the design.
| Epitope | Origin | Position(aa) | Epitope type | Synthesized epitope genes and used primers(5′-3′) in PCRa |
|---|---|---|---|---|
| F1 | S1 | 24–150 | B (neutrallization) | S: GTA GGATCCCCACCATGG ATAATTATGTG |
| A: TCA GGGCCC ATTATAAAATAGA | ||||
| F2 | S1 | 240–255 | B | 5′ CAATACTGGTAATTTTTCAGATGGGTTTTACC |
| CTTTTACTAATTCTAGTGGCGC 3′ | ||||
| F3 | S1 | 290–400 | B (neutrallization) | S: ACT GGCGCC CAAGGTGGTGTTCA |
| A: AGT GGGCCC TTCAAAATTTTGTC | ||||
| F4 | S1 | 532–537 | T | 5′ CATTAAACTCACTAAAGAGGGT AACGTT GGCGC 3′ |
| F5 | S2 | 1–65 | B (neutralization) | S: ACT GGCGCC TCTACTAGTGAAAATG |
| T(CTL) A: AGT GGGCCC ACGTGTTTGTATGTA | ||||
| F6 | N | 1–120 | T (CTL) | S: AGT GGGCCC GCAAGCAGTAAGGCA |
| B A: ACT GGCGCC ACCCTTAGCAGCA | ||||
| F7 | N | 290–410 | T (CTL) | S: AGT GGCGCC AAGCTTCAACCTGA |
| A: ACT CTCGAG TTATTAGAGTTCATTTTC |
a
Note. Letters in italic means restriction sites, underlined means Kozak sequence, bloded means CpG motif. ‘S′ means sense primer, while ‘A′ means anti-sense primer.