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. 2006 Jul 11;347(4):994–1000. doi: 10.1016/j.bbrc.2006.06.190

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Copyright © 2006 Elsevier Inc. All rights reserved.

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Fig. 3 — WNVCp binds to the substrate binding domain of Hsp70. (A) Reaction mixtures containing Hsp70 (0.5 μM) + His-WNVCp (4 μM) (lanes 1 and 2), ATPase (1 μM) + His-WNVCp (4 μM) (lanes 3 and 4), and SBD (1 μM) + His-WNVCp (4 μM) (lanes 5 and 6) were incubated (T: lanes 1, 3, and 5), and protein complexes were precipitated (P: lanes 2, 4, and 6) by Ni²⁺–agarose. (B) Reaction mixtures containing Hsp70 (1 μM) + His-WNVCp (5 μM) (lanes 1 and 2) and Hsp70AAAA (1 μM) + His-WNVCp (5 μM) (lanes 3 and 4) were incubated (T: lanes 1 and 3), and protein complexes were precipitated (P: lanes 2 and 4) by Ni²⁺–agarose. Precipitated proteins were analyzed as described in Fig. 1B.