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. 2005 Sep 26;337(2):720–729. doi: 10.1016/j.bbrc.2005.09.098

Fig. 1.

Fig. 1

Misexpression of the SARS-CoV 3a gene in Drosophila. (A,B) Misexpression of the SARS-CoV 3a gene caused external eye disruption in adult Drosophila. Misexpression of the control EGFP transgene (A) showed normal external eye morphology, whereas misexpression of the SARS-CoV EGFP-3a transgene (B) caused a rough eye phenotype as characterized by loss of regularity of the external eye structure. (C–G) Subcellular localization of the EGFP-3a fusion protein in Drosophila. Misexpression of the EGFP control protein showed homogeneous intracellular green fluorescence signals in both third instar eye imaginal disc (C) and salivary gland (E) cells. A distinct punctate cytoplasmic expression pattern of the EGFP-3a fusion protein was observed in both eye imaginal disc (D) and salivary gland cells (F). (G) Location of salivary gland cell nuclei of (F). Propidium iodide (PI) was used to stain cell nuclei in eye imaginal disc cells (C,D).