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. 2005 Sep 26;337(2):720–729. doi: 10.1016/j.bbrc.2005.09.098

Fig. 3.

Fig. 3

Apoptotic pathway is related to the EGFP-3a-induced rough eye phenotype. (A–F) Overexpression of anti-apoptotic genes suppresses the EGFP-3a-induced rough eye phenotype. gmr-GAL4 alone (A) and misexpression of EGFP-lacZ fusion protein by gmr-GAL4 (B) showed no external eye deformation. Overexpression of the pro-apoptotic gene reaper caused a rough eye phenotype and reduction in eye size (C). The EGFP-3a-induced rough eye phenotype (D) was suppressed when coexpressed with either P35 (E) or DIAP1 (F). (G–M) Coexpression of anti-apoptotic genes reduces the number of apoptotic cells in third instar larval eye discs. Comparable numbers of acridine orange-positive apoptotic cells were observed in gmr-GAL4 control eye discs (G) and discs misexpressed with a GFP-lacZ fusion protein (H). Expression of both reaper (I) and EGFP-3a (J) showed increased number of acridine orange-positive cells in eye discs. The EGFP-3a-induced apoptosis was suppressed by coexpression of baculoviral anti-apoptotic genes P35 (K), DIAP1 (L); and cytochrome c dc3 (M). Arrows indicate the location of the morphological furrows.