Fig. 5.

EGFP-3a interacts with gene transcription regulatory machineries. Misexpression of the SARS-CoV 3a gene disrupted the normal external eye structure (A; gmr-GAL4) and caused a rough eye phenotype in Drosophila (B). The EGFP-3a-induced rough eye phenotype was dominantly suppressed by a chromosome deletion Df(3R)Exel8157 (C) which uncovers the 87D8-10 region, and by a null allele of C-terminal-binding protein (CtBP^87De-10) (D). The EGFP-3a-induced rough eye phenotype was suppressed by the Ubc9 mutant lesswright (lwr⁰²⁸⁵⁸) which is an E2 enzyme that SUMOylates CtBP (E). A P-element allele of Suppressor of hairless (Su(H)^EY07695), inserted in the 5′ untranslated region of Su(H), dominantly suppressed EGFP-3a-induced rough eye phenotype (F).