TABLE 1.
List of known or probable flagellar membrane proteins in T. brucei, Leishmania species, and T. cruzia
| Gene ID | Name(s) | Reference(s) | Comments |
|---|---|---|---|
| (A) Flagellar membrane proteins identified by fluorescence microscopy | |||
| Tb927.8.4050/4100 | FLA1BP | 39 | PF, 1 TM, FAZ attachment |
| Tb927.5.4570/4580 | FLA1BP | 40 | BF, 1 TM, FAZ attachment |
| Tb927.11.17040, 10.16190, 10.13040, 11.13740, respectively | ACP1, ACP2, ACP4, ACP5, respectively | 78 | PF, 1 TM, adenylate cyclases |
| Tb927.4.3750 | ESAG4 | 98 | BF, 1 TM, adenylate cyclase |
| Tb927.2880 | FS179, TbCaCh | 42, 43 | BF, 24 TM, putative Ca2+ channel |
| Tb927.8.6010 | TbHrg | 128 | PF and BF, 4 TM, heme transporter |
| Tb927.6.1520 | TbAQP1 | 129 | PF and BF, 6 TM, aquaporin |
| Tb927.7.4270 | Hypothetical protein | 120 | PF and BF, 1 TM |
| Tb927.11.1830 | Hypothetical protein | 120 | PF and BF, 6 TM |
| Tb927.1.2120 | TbCALP1.3 | 130 | PF and BF, dual acylation, calpain-like protein |
| Tb927.1.2160 | TbSKCRP1.5 | 130 | PF and BF, dual acylation, calpain-like protein |
| Tb927.9.6170 | Arginine kinase | 122 | PF and BF, dual acylation |
| TcCLB.509391.20 | FCaBP | 101 | All life cycle stages, dual acylation, flagellar Ca2+ binding protein |
| LmxM.36.6300 | GT1 | 110 | Glucose transporter, potential glucose sensor |
| LmjF.20.1310 | SMP-1 | 108 | Myristolyated protein that stabilizes the flagellar membrane |
| LinJ.31.0910 | AAP3 | 82 | Arginine transporter, potential arginine sensor |
| LmjF.31.0020 | AQP1 | 96 | Involved in drug sensitivity, osmotic response |
| (B) Flagellar membrane proteins in L. mexicana promastigotes identified by proteomics | |||
| LmxM.23.0840 | Hypothetical protein | 121 | 1 TM |
| LmxM.04.0630 | Hypothetical protein | 121 | 1 TM |
| LmxM.08_29.2440 | Cyclic nucleotide phosphodiesterase | 121 | 1 TM |
| LmxM.27.0970 | ABC transporter, putative | 121 | 14 TM |
| LmxM.07.0830 | Hypothetical protein | 121 | 3 TM |
| LmxM.12.0891 | Surface antigen protein 2 | 121 | 1 TM |
| LmxM.29.0850 | Surface protein amastin, putative | 121 | 4 TM |
| LmxM.36.3180 | Receptor-type adenylate cyclase A-like protein | 121 | 1 TM |
| LmxM.04.0190 | Surface antigen-like protein | 121 | 1 TM |
| LmxM.33.0910 | Hypothetical protein | 121 | 1 TM |
| LmxM.36.2590 | Membrane-bound acid phosphatase 2, putative | 121 | 2 TM |
| LmxM.25.2090 | Hypothetical protein | 121 | 1 TM |
| LmxM.10.0390 | GP63, leishmanolysin | 121 | 1 TM |
| LmxM.05.1030 | Hypothetical protein | 121 | 1 TM |
| LmxM.36.2710 | Mitochondrial ATP-dependent zinc metallopeptidase | 121 | 1 TM |
| LmxM.32.0900 | DnaJ chaperone-like protein | 121 | 8 TM |
| LmxM.30.0350 | AATP11, putative amino acid transporter | 121 | 11 TM |
| LmxM.30.2310 | 3′-Nucleotidase/nuclease | 121 | 2 TM |
Many of the proteins in Part A were localized by fluorescence microscopy in the referenced publications, and a few of the T. brucei flagellar membrane proteins were identified by TrypTag (120) as flagellar proteins with predicted TM domains. Part B lists proteins from the flagellar proteome of L. mexicana (121). This list includes all proteins that met the following criteria: ≥1 predicted transmembrane domain; log2 solubility in detergent soluble fraction >0; and enriched in the flagellar versus cell body fraction by log2 ≥ 1. PF and BF refer to expression detected in procyclic forms or bloodstream forms, and TM indicates the number of predicted transmembrane domains.