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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Methods Mol Biol. 2020;2117:271–284. doi: 10.1007/978-1-0716-0301-7_17

Fig. 2. A procedure for fibroblast plating.

Fig. 2

To optimize the efficiency of reprogramming, fibroblasts are plated at multiple densities ranging from 3,000 to 30,000 cells per well. The total plating volume after addition of plating medium (a) and resuspended fibroblasts (b) equals 2 mL