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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Methods Mol Biol. 2020;2117:271–284. doi: 10.1007/978-1-0716-0301-7_17

Fig. 3. Visual representation of the reprogramming protocol.

Fig. 3

The full reprogramming procedure takes approximately 20 days to pick iPSC colonies for expansion (a). 10,000 fibroblasts plated into one well of a 6-well plate appear relatively sparse on the first day of transfection (b). Following the first transfection, fibroblasts adopt a more compact morphology with fewer cellular projections (c,d). By days 6–7, cells begin to rapidly proliferate (e) and should become confluent (f). Colonies may first be observed on day 12 following the sixth transfection (g) and will grow larger and more clearly distinguishable by day 18 (h-j). Dashed line indicates an iPSC colony. Scale bar = 50 μm