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. 2011 Jan 21;43(3):161–171. doi: 10.1093/abbs/gmq127

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© The Author 2011. Published by ABBS Editorial Office in association with Oxford University Press on behalf of the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

This article is made available via the PMC Open Access Subset for unrestricted re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the COVID-19 pandemic or until permissions are revoked in writing. Upon expiration of these permissions, PMC is granted a perpetual license to make this article available via PMC and Europe PMC, consistent with existing copyright protections.

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Antibody microarray for protein profiling and comparison The key for antibody microarrays is the quality of the antibodies. Antibodies could be either commercially obtained or home-made. They must pass the strict quality control step of both specificity and efficacy prior to microarray fabrication. The protein profiling part is similar to the gene expression microarray. In brief, the paired samples (treated/untreated or patient/health) were labeled with Cy3 and Cy5, respectively. Then the labeled samples were mixed at equal amounts and probed on the antibody microarray. The pseudo-color represents the ratio of a given protein between sample A and sample B.