Fig 6. Transient complementation allows viP₁₁A6L and viP₁₁A3L replication in the absence of DOX.

BS-C-1 cell monolayers were infected with viP₁₁A6L (A) or viP₁₁A3L (B) at an MOI of 0.01 in the absence of DOX and transfected with plasmids expressing the A6L (pP₁₁A6L) or A3L (pP₁₁A3L) genes under the constitutive VACV P₁₁ promoter, or no plasmid (mock). Infections were also performed in the presence of 1 μg/ml DOX (DOX). Cells were collected immediately after infection (input, dotted line) or 2 DPI. Virus yield was determined by plaque assay on BS-C-1 cells in the presence of 1 μg/ml DOX. The data shown represent the mean viral yields from triplicate samples assayed in duplicate. Error bars indicate standard deviation. Data are representative of two separate experiments.