. 2020 Apr 2;15(4):e0230711. doi: 10.1371/journal.pone.0230711

Table 2. Primers used to amplify wild-type and recombinant VACV genomic regions.

Primer	Gene	Sequence (5’ to 3’)
1	tetR	`GACGCCTTAGCCATTGAGAT`
2	EGFP	`ACAACCACTACCTGAGCACC`
3	E7R	`TCTCCGCACATGGAACTCAT`
4	E8R	`CAGAGAACGATCCATTAGCA`
5	A3L	`GATGCTACTTCGTCGATGGA`
6	A4L	`CAAATCCAGGAGCAGCATCT`
7	A6L	`TATCAACATCTGATGCGCT`
8	A7L	`ATGTTATTGCGTCTGATGCC`
9	I8R (forward)	`ATTTTCCAATTCCGTAGGTAAACGA`
10	I8R (reverse)	`TGATCATGCTCATGAACTTCGTCTA`
11	A6L (forward)	`TAAATACGGCCGATGGACAAACTTAG`
12	A6L (reverse)	`ACGATAGCTAGCTTAGAATTTATACG`
13	A3L (forward)	`TAAATACGGCCGATGGAAGCCGTG`
14	A3L (reverse)	`CATAATGCTAGCCTAAAATAGTTC`