Table 1. Sequences and physical information of 28 SSR markers used for PCR amplification.
Nam | Allele size (base-pairs) | Forward Primer 5'- 3' | Reverse primer 3'-5' | Annealing temperature (Tm: oC) |
---|---|---|---|---|
ADL0268 | 102–116 | CTCCACCCCTCTCAGAACTA | CAACTTCCCATCTACCTACT | 60 |
MCW0206 | 221–249 | ACATCTAGAATTGACTGTTCAC | CTTGACAGTGATGCATTAAATG | 60 |
LEI0166 | 354–370 | CTCCTGCCCTTAGCTACGCA | TATCCCCTGGCTGGGAGTTT | 60 |
MCW0295 | 88–106 | ATCACTACAGAACACCCTCTC | TATGTATGCACGCAGATATCC | 60 |
MCW0081 | 112–135 | GTTGCTGAGAGCCTGGTGCAG | CCTGTATGTGGAATTACTTCTC | 60 |
MCW0014 | 164–182 | TATTGGCTCTAGGAACTGTC | GAAATGAAGGTAAGACTAGC | 58 |
MCW0183 | 296–326 | ATCCCAGTGTCGAGTATCCGA | TGAGATTTACTGGAGCCTGCC | 58 |
ADL0278 | 114–126 | CCAGCAGTCTACCTTCCTAT | TGTCATCCAAGAACAGTGTG | 60 |
MCW0067 | 176–186 | GCACTACTGTGTGCTGCAGTTT | GAGATGTAGTTGCCACATTCCGAC | 60 |
MCW0104 | 190–234 | TAGCACAACTCAAGCTGTGAG | AGACTTGCACAGCTGTGTACC | 60 |
MCW0123 | 76–100 | CCACTAGAAAAGAACATCCTC | GGCTGATGTAAGAAGGGATGA | 60 |
MCW0330 | 256–300 | TGGACCTCATCAGTCTGACAG | AATGTTCTCATAGAGTTCCTGC | 60 |
MCW0165 | 114–118 | CAGACATGCATGCCCAGATGA | GATCCAGTCCTGCAGGCTGC | 60 |
MCW0069 | 158–176 | GCACTCGAGAAAACTTCCTGCG | ATTGCTTCAGCAAGCATGGGAGGA | 60 |
MCW0248 | 205–225 | GTTGTTCAAAAGAAGATGCATG | TTGCATTAACTGGGCACTTTC | 60 |
MCW0111 | 96–120 | GCTCCATGTGAAGTGGTTTA | ATGTCCACTTGTCAATGATG | 60 |
MCW0020 | 179–185 | TCTTCTTTGACATGAATTGGCA | GCAAGGAAGATTTTGTACAAAATC | 60 |
MCW0034 | 212–246 | TGCACGCACTTACATACTTAGAGA | TGTCCTTCCAATTACATTCATGGG | 60 |
LEI0234 | 216–364 | ATGCATCAGATTGGTATTCAA | CGTGGCTGTGAACAAATATG | 60 |
MCW0103 | 266–270 | AACTGCGTTGAGAGTGAATGC | TTTCCTAACTGGATGCTTCTG | 64 |
MCW0222 | 220–226 | GCAGTTACATTGAAATGATTCC | TTCTCAAAACACCTAGAAGAC | 60 |
MCW0016 | 162–206 | ATGGCGCAGAAGGCAAAGCGATAT | TGGCTTCTGAAGCAGTTGCTATGG | 60 |
MCW0037 | 154–160 | ACCGGTGCCATCAATTACCTATTA | GAAAGCTCACATGACACTGCGAAA | 64 |
MCW0098 | 261–265 | GGCTGCTTTGTGCTCTTCTCG | CGATGGTCGTAATTCTCACGT | 60 |
LEI0094 | 247–287 | GATCTCACCAGTATGAGCTGC | TCTCACACTGTAACACAGTGC | 60 |
MCW0284 | 235–243 | GCCTTAGGAAAAACTCCTAAGG | CAGAGCTGGATTGGTGTCAAG |
60 |
MCW0078 | 135–147 | CCACACGGAGAGGAGAAGGTCT | TAGCATATGAGTGTACTGAGCTTC | 60 |
LEI0192 | 244–370 | TGCCAGAGCTTCAGTCTGT | GTCATTACTGTTATGTTTATTGC | 60 |
ADL0112 | 120–134 | GGCTTAAGCTGACCCATTAT | ATCTCAAATGTAATGCGTGC | 58 |
MCW0216 | 139–149 | GGGTTTTACAGGATGGGACG | AGTTTCACTCCCAGGGCTCG | 60 |
Source: FAO [32]