Table 1. List of primers (5′-3′) used to amplify hsp70 with and without intron, hsc70-I, hsc70-II, and actin in RT-PCR analysis.
| Primer | Application | Sequence (5ʹ– 3ʹ) |
|---|---|---|
| Deg_Hsp70-F | 5ʹ-3ʹ RACE PCR | ACVGNTCCNGCNTAYTTYAAYGA |
| Deg_Hsp70-R | 5ʹ-3ʹ RACE PCR | GCNACNGCYTCRTCNGGRTT |
| GSP-HSP70_F | 5ʹ RACE PCR | TGATGCAAAGCGGCTGATTGGACGTA |
| GSP-HSp70_R | 3ʹ RACE PCR | TTGGCAGCATCTCCAATTAATCTTTCTGTATC |
| GSP-HSC70I_F | 5ʹ RACE PCR | GTCGTAAATTCGATGACCCC |
| GSP-HSC70I_F HSC70I_R | 3ʹ RACE PCR | GACATTACGTTCTCCAGCAG |
| GSP-HSC70II_F | 5ʹ RACE PCR | GTTTGATCGGTCGTGAATGGAG |
| GSP-HSC70II_R | 3ʹ RACE PCR | CCCAAATGTGTGTCACCGTTT |
| Hsp70 Forward | Expression (Fig 2A) |
TTGGGAACAACATATTCCTGC |
| Hsp70 Reverse | Expression (Fig 2A) |
TTCGTTTAGCATCAAAGACACTG |
| Hsc70I Forward | qPCR (Fig 3B and 3C) | GTCGTAAATTCGATGACCCC |
| Hsc70I Reverse | qPCR (Fig 3B and 3C) | GACATTACGTTCTCCAGCAG |
| Hsc70II Forward | qPCR (Fig 3B and 3C) | GTTTGATCGGTCGTGAATGGAG |
| Hsc70II Reverse | qPCR (Fig 3B and 3C) |
CCCAAATGTGTGTCACCGTTT |
| Hsp70 tot F | qPCR (Fig 3B and 3C; Fig 4A, 4B and 4C) | TGTTGGAGTTTATCAACATGGA |
| Hsp70 tot R | qPCR (Fig 3B and 3C; Fig 4A, 4B and 4C) | TTTGGCAGCATCTCCAATTA |
| Hsp70 genomic F | gDNA amplification (Fig 2B and 2C) |
GAAACAGAACAACACCCAGCT |
| Hsp70 genomic R | gDNA amplification (Fig 2B and 2C) | ACTTCAGCAGTTTCACGCAT |
| Actin Forward | qPCR (Fig 3B and 3C; Fig 4C and 4D, 4E and 4F) | CTGCCTCAACCTCATTGGAAAA |
| Actin Reverse | qPCR (Fig 3B and 3C; Fig 4A, 4B and 4C) | TGGTTGTAGACGGTTTCGTG |