Table 10. Specificity (%) and 95% confidence intervals (95% CI) of Kalazar Detect performed at the point of care (POC) and IT-Leish and other tests performed at IMT, in serum samples from asymptomatic and potential cross-reactive controls, according to the collection site.
| Locality (n) | Specificity% (n) | ||||
|---|---|---|---|---|---|
| CI 95% | |||||
| KD-POC | KD-IMT | IT-Leish | IFA | ELISA | |
| Campo Grande (38) | 94.7 (36) | 89.5 (34) | 97.4 (37) | 97.4 (37) | 55.3 (21) a |
| 82.7–98.5 | 75.9–95.8 | 86.5–99.5 | 86.5–99.5 | 39.7–69.8 | |
| Bauru (7) | 100.0 (7) | 100.0 (7) | 100.0 (7) | 100.0 (7) | 71.4 (5) |
| 64.6–100.0 | 64.6–100.0 | 64.6–100.0 | 64.6–100.0 | 35.9–91.8 | |
| Aracaju (13) | 92.3 (12) | 84.6 (11) | 84.6 (11) | 76.9 (10) | 38.5 (5) b |
| 63.7–98.6 | 57.8–95.7 | 57.8–95.7 | 49.7–91.8 | 17.7–64.5 | |
| Natal (33) | 100.0 (33) | 97.0 (32) | 100.0 (33) | 84.8 (28) | 60.6 (20) c |
| 89.6–100.0 | 84.7–99.5 | 89.6–100.0 | 69.1–93.3 | 43.7–75.3 | |
| Sao Paulo (15) | 100.0 (15) | 100.0 (15) | 100.0 (15) | 73.3 (11) | 26.7 (4) d |
| 79.6–100.0 | 79.6–100.0 | 79.6–100.0 | 48.0–89.1 | 10.9–51.9 | |
| Total (106) | 97.2 (103) | 93.4 (99) | 97.2 (103) | 87.7 (93) | 51.2 (55) e |
| 92.0–99.0 | 87.0–96.8 | 92.0–99.0 | 80.1–92.7 | 42.5–61.2 | |
n–number of samples.
KD-POC–Kalazar Detect performed at point of care.
KD-IMT–Kalazar Detect processed at IMT.
IT-Leish–rK39 –RDT.
IFA–L. major-like based Indirect immunofluorescence assay.
ELISA–L. major-like based Enzyme-linked immunosorbent assay.
No difference across localities (chi-square, p>0.05).
a–p = 0.002,
b–p = 0.041,
c–p = 0.001,
d–p = 0.003,
e–p<0.001 (McNemar’s test) in relation to KD-IMT.