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. 2020 Apr 2;15(4):e0230943. doi: 10.1371/journal.pone.0230943

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© 2020 Lee et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 1 — (A) The genome structure of cspg4 in zebrafish (ENSDART00000112782) compared to human (ENST00000308508), rat (ENSRNOT00000023326.3), mouse (ENSMUST00000035661) and medaka (ENSORLT00000002266.1). Scheme demonstrates the target sites of splicing (MO-e2i2) and translational blocking (MO-start) morpholino (black line) in zebrafish. Gray lines indicate primer pairs used to evaluate pre-mRNA processing. (B) The protein sequence of Cspg4 among zebrafish (UniProt # E7FCN9), rat (UniProt # F1LS79), human (UniProt # Q6UVK1), mouse (UniProt # Q8VHY0) and medaka (UniProt # H2L8T3) were analyzed by Jalview2 software with PAM250 matrix and the Neighbour Joining method [37].