Skip to main content
. 2020 Apr 2;15(4):e0230943. doi: 10.1371/journal.pone.0230943

Fig 4. Cspg4 morphants had abnormal pharygeal cartilage at 3 dpf.

Fig 4

The cartilage was stained by using Alcian blue staining. (A, B) The ventral view and lateral view of 4 ng control MO injected embryo at 3 dpf. (C, D) Four ng cspg4 MO-e2i2 injected morphants had disrupted Meckel’s cartilage (arrow head) and underdeveloped ceratohyal cartilage (arrow). (E, F) The phenotypes of abnormal pharyngeal cartilage development were rescued by co-injecting 250 pg cspg4 mRNA with 4 ng MO-e2i2. The number in bottom right indicates the penetrance of phenotype.