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. 2020 Apr 2;15(4):e0230943. doi: 10.1371/journal.pone.0230943

Fig 6. Cspg4 transmembrane domain mutant embryo phenocopied cspg4 overexpression phenotype.

Fig 6

(A) Scheme illustrates wild-type and mutant Cspg4 protein structure. (B) The flag-tag signal of both wild-type and mutant Cspg4 were detected in the cell lysate, but in culture medium the signal of flag-tag was only detected in mutant Cspg4-flag transfected group. (C) Eight percent of cspg4 em1twu0405 embryos were cyclopia. (D) Four percent of cspg4 em1twu0405 embryos showed midline bifurcation (notochord was indicated by asterisk).