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. 2020 Apr 2;15(4):e0230943. doi: 10.1371/journal.pone.0230943

Fig 7. The phenotypes of cspg4 morphants were rescued by co-injecting wnt11 mRNA.

Fig 7

(A) Wnt11 had binding affinity with Cspg4. Wnt11-HA (indicated by arrow) was co-precipitated with Cspg4-flag. Asterisk mark (*) indicates non-specific band. (B) The abnormal body axis formation phenotype in cspg4 morphants (4ng MO-e2i2) was rescued by co-injecting 25 pg wnt11 mRNA. Yellow lines indicate the angle between anterior end and tailbud. (C) Co-injecting 25 pg wnt11 mRNA with 4 ng cspg4 morpholino rescued the short body length phenotype. (D) The angle between anterior end and tailbud was reduced in wnt11 rescue group compared to cspg4 knockdown group (control, n = 41; cspg4 knockdown, n = 89; wnt11 rescue, n = 117). (E) The body length of wnt11 rescue group at 1 dpf was significantly longer than cspg4 knockdown group (control, n = 40; cspg4 knockdown, n = 66; wnt11 rescue, n = 109). The significant difference was tested using ANOVA followed by Tukey’s multiple comparison. Different letters (a, b or c) indicate significant difference between groups (p ≤ 0.05). Data are presented as mean ± SD.