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. 2020 Mar 23;18(3):e3000671. doi: 10.1371/journal.pbio.3000671

Fig 5. Response of CsIVP-RNAi plants to pathogen infection.

Fig 5

(A) Increased tolerance of CsIVP-RNAi plants to pathogen attack during growth under greenhouse conditions. (B) Disease level of WT and CsIVP-RNAi plants under greenhouse and growth chamber conditions. (C–E) Phenotypic characterization of WT and CsIVP-RNAi plants 11 dpi with Pseudoperonospora cubensis in a growth chamber. The second, third, and fourth leaves (from bottom to top) are displayed in panel E. (F–G) Sporulation of P. cubensis on WT and CsIVP-RNAi plants imaged by scanning electron microscopy. (H) PsITS expression after inoculation with P. cubensis at 0, 3, 7, and 11 dpi. (I) CsIVP expression after inoculation with P. cubensis at 0, 3, 7, and 11 dpi. Scale bars represented 2 cm in panels A and C–E and 50 μm in panels F–G. Values are means ± SE (n = 3) in panels H–I. Asterisk indicate significant difference at P < 0.05 by t test. The data underlying this figure are included in S4 Data. CsIVP, Cucumis sativus Irregular Vasculature Patterning; dpi, days post inoculation; PsITS, P. cubensis internal transcribed spacer; RNAi, RNA interference; WT, wild type.