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. 2020 Mar 23;16(3):e1008403. doi: 10.1371/journal.ppat.1008403

Fig 4. MuPyV DNA progresses from LT to focal RPA32 subdomains.

Fig 4

MEFs were infected with WT MuPyV and processed and labeled as described in Fig 3. Cells were stained for RPA32 (green), LT (red), and EdU-labeled vDNA (gray), then imaged by 3D-SIM. A single z-plane of representative nuclei from: (A) 0 min (no chase), (B) 30 min, and (C) and 60 min chase conditions. Arrowheads in each panel indicate regions of vDNA (EdU) localization relative to RPA32 and LT. Scale Bars: Nucleus = 5μm, Crop = 0.5μm. (D) PCC analysis of vDNA (EdU) with either LT, dim RPA32, or focal RPA32. Unpaired t-tests were used to compare mean values (** = p<0.01). n = 6 nuclei per time point.