Fig 2. Locations of eDNA sampling sites in the Mbeya Region of Tanzania in 2018.

In total eight sites were surveyed, labelled A-H. The key results from eDNA survey (helix symbol), host snail survey (snail symbol) and test for the presence of schistosomes in snail tissue (cercariae symbol) are given for S. haematobium (H) and S. mansoni (M) assays, with + indicating the site was positive, while a dot indicates no detection was made. Map drawn using the following open source software and data: DIVA-GIS7.5 (https://www.diva-gis.org); Africa river network (15 sec resolution), African drainage basin (15sec resolution) and African Digital Elevation Model data (30 sec resolution) from HydroSHEDS (https://www.hydrosheds.org); African Water Bodies shapefile from RCMRD GeoPortal (http://geoportal.rcmrd.org).