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. 2020 Mar 23;14(3):e0008129. doi: 10.1371/journal.pntd.0008129

Fig 3. Probability of qPCR amplification of DNA standards of concentrations ranging from 1 copy/μl to 1,000,000 copies/μl.

Fig 3

a) S. mansoni assay–probability of amplification in any one qPCR across all standard templates tested. b) S. mansoni assay–probability of amplification in any one standard template that is subject to triplicate qPCR, c) S. haematobium assay–probability of amplification in any one qPCR across all standard templates tested, d) S. haematobium assay–probability of amplification in any one standard template that is subject to triplicate qPCR. Lines represent logistic models of the form y = a / (1 + b*e(-cx)). a) a = 1.002, b = 27.303, c = 3.826. b) a = 1.000, b = 7.501, c = 4.786. c) a = 1.004, b = 1.009, c = 1.415. b) a = 1.004, b = 0.342, c = 1.399. All models r > 0.995. Grey shading indicates 95% confidence intervals.