Table 4. Detection of S. mansoni DNA from aquarium water samples.
| Status | Replicate | No. snails (individuals) |
Water filtered (ml) | DNA concentration ng per μl |
Amplification success |
Ct mean (cycle) |
Mean copies per μl (range) |
|---|---|---|---|---|---|---|---|
| Non-infected | Tank 1: Rep 1 | 33 | 500 | 54.95 | 0/3 | - | - |
| Tank 1: Rep 2 | 460 | 58.50 | 0/3 | - | - | ||
| Tank 2: Rep 1 | 28 | 500 | 115.00 | 0/3 | - | - | |
| Tank 2: Rep 2 | 460 | 61.3 | 0/3 | - | - | ||
| Infected | Tank 3: Rep 1 | 19 | 160 | 84.50 | 3/3 | 26.95 | 9041 (8580 to 9949) |
| Tank 3: Rep 2 | 90 | 63.65 | 3/3 | 27.77 | 9011 (8392 to 9958) | ||
| Tank 4: Rep 1 | 75 | 200 | 85.90 | 3/3 | 27.19 | 7565 (7342 to 7737) | |
| Tank 4: Rep 2 | 200 | 114.10 | 3/3 | 26.34 | 14038 (13384 to 14518) | ||
| Tank 5: Rep 1 | 13 | 70 | 88.95 | 3/3 | 25.58 | 24599 (22498 to 28659) | |
| Tank 5: Rep 2 | 60 | 102.20 | 3/3 | 25.39 | 28130 (26232 to 30259) | ||
| Tank 6: Rep 1 | 18 | 73 | 100 | 3/3 | 25.15 | 33296 (33066 to 33739) | |
| Tank 6: Rep 2 | 78 | 114.30 | 3/3 | 24.64 | 48264 (45033 to 52181) | ||
| Negative control | NA | 0 | 500 | - | 0/3 | - | - |