Skip to main content
. Author manuscript; available in PMC: 2020 Jul 13.
Published in final edited form as: Nat Chem Biol. 2020 Jan 13;16(2):170–178. doi: 10.1038/s41589-019-0445-9

Figure 2. Assigning DAG substrate specificity to the DGK superfamily.

Figure 2.

a) Heat map displaying live cell alterations in the DAG lipidome (log2 fold change) in recombinant human DGK overexpressed- compared with mock non-transfected-HEK293T cells (n=5 biological samples). All 10 isoforms from the DGK superfamily were analyzed by targeted lipidomics. b) Heat map highlighting altered DAGs that were unique and/or shared across DGK isoforms that showed at least a 15% change (log2 fold change of −0.23) in cellular lipid abundance (n=5 biological samples). Note that C18:0_C22:6 (DGKζ2) and C16:0_C22:6 (DGKε) DAGs showed changes at the set cutoff (~0.23). All data shown are representative of two experiments (n=2 biologically independent experiments).